Every calculator you need for Biomedical Resarch

Cell Doubling Time Calculator (DT) – Calculate Culture Growth Rates

Quickly determine the growth rate of your cell culture. This tool calculates the Doubling Time (DT) based on cell counts taken at two different time points.
Just enter your initial cell count (N1) and the time it was taken (T1), followed by your final count (N2) and time (T2). The calculator uses the logarithmic growth formula to provide the precise doubling time in hours.
If you have any questions or confusions about the topic, ask Sophie AI!

Initial Time (Hours)

Initial Cell Count

Final Time (Hours)

Final Cell Count

Doubling Time

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Read Our Article on Cell Doubling Time Calculaion!

Cell Seeding & Mix Calculator – Hemocytometer Counting to Seeding

This all-in-one calculator takes your raw hemocytometer counts and generates a complete "Master Mix" recipe for seeding multi-well plates.

Select your plate type (e.g., 48-well).
Enter the raw counts from your hemocytometer squares (1–4) and your dilution factor.
Enter your desired target cells per well.
Result: You get a specific recipe telling you exactly how much cell stock and media to combine, and the precise volume to dispense per well.

If you have any questions or confusions about the topic, ask Sophie AI!

Volume per Well (mL)

Desired Cell Number per Well

Count Per Square 1

Count Per Square 2

Count Per Square 3

Count Per Square 4

Dilution Factor

Cell Seedng Density

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Read Our Article to understand these numbers!

IC50 & EC50 Calculator – Dose-Response & Inhibition Analysis

This tool performs linear interpolation to find the exact concentration required for 50% inhibition (IC50) or 50% effect (EC50). It automatically detects whether your compound is an inhibitor or an agonist.

Enter your desired concentration unit, average Blank signal (background) and average Negative Control signal (Concentration 0 : 100% viability).
Select how many concentration points you tested (2–12).
Input the concentration and average signal for each point.
Result: The tool calculates the exact IC50/EC50 value and reports if the potency is outside your tested range.

If you have any questions or confusions about the topic, ask Sophie AI!

Concentration Unit

Number of Concentrations

Average Blank Signal

Avg Neg Control (Conc 0)

Conc 1

Data 1

Conc 2

Data 2

Conc 3

Data 3

Conc 4

Data 4

Conc 5

Data 5

Conc 6

Data 6

Read Our Article to understand these numbers!

Conc 7

Data 7

Conc 8

Data 8

Conc 9

Data 9

Conc 10

Data 10

Conc 11

Data 11

Conc 12

Data 12

IC50/EC50 Result

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Solution Conversions Calculator: Molarity, Dilutions & Percentages

Whether you are making a molar stock from powder, preparing a percentage solution (w/v), or calculating a dilution series, this tool provides step-by-step recipes:

Select your calculation mode (e.g., Molarity, Dilution, or Dilution Factor).
Fill in the required fields (Molecular Weight, Target Volume, etc.).
Result: Receive a clear instruction, such as "Dissolve 5g in 80mL of water, then QS to 100mL," or "Mix 1 part sample with 9 parts buffer."

If you have any questions or confusions about the topic, ask Sophie AI!

Formula Weight (MW) g/mol

Desired Conc [mM]

Desired Volume [mL]

Results

Standard Curve Calculator – Linear Regression & Concentration Analysis

This tool generates a linear regression line (y = mx + b) from your standards and automatically calculates the concentration of your unknown samples, including Standard Deviation adjustments.

Enter your Concentration unit an dilution factor.
Select the number of Standards and Unknown Samples you have.
Enter the concentration and average signal (Data) for your standards to generate the curve. (Notice, first concentration Conc1 is 0 as your blank)
Enter the average data and the Standard Deviation (SD) for your samples.
Result: View a real-time scatter plot of your standard curve and a bar graph of your sample concentrations.

If you have any questions or confusions about the topic, ask Sophie AI!

Conc Unit

Num of Standards

Num of Samples

Dilution Factor

Conc 1

Data 1

Conc 2

Data 2

Conc 3

Data 3

Conc 4

Data 4

Conc 5

Data 5

Conc 6

Data 6

Conc 7

Data 7

Conc 8

Data 8

Smp1 Data

Standard Dev

Smp2 Data

Standard Dev

Smp3 Data

Standard Dev

Smp4 Data

Standard Dev

Smp5 Data

Standard Dev

Smp6 Data

Standard Dev

Smp7 Data

Standard Dev

Smp8 Data

Standard Dev

Sample Concentrations

Every calculator you need for Biomedical Resarch

Cell Doubling Time Calculator (DT) – Calculate Culture Growth Rates

-- Hrs

Cell Seeding & Mix Calculator – Hemocytometer Counting to Seeding

This all-in-one calculator takes your raw hemocytometer counts and generates a complete "Master Mix" recipe for seeding multi-well plates.

Select your plate type (e.g., 48-well).

Enter the raw counts from your hemocytometer squares (1–4) and your dilution factor.

Enter your desired target cells per well.

Result: You get a specific recipe telling you exactly how much cell stock and media to combine, and the precise volume to dispense per well.

If you have any questions or confusions about the topic, ask Sophie AI!

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IC50 & EC50 Calculator – Dose-Response & Inhibition Analysis

This tool performs linear interpolation to find the exact concentration required for 50% inhibition (IC50) or 50% effect (EC50). It automatically detects whether your compound is an inhibitor or an agonist.

Enter your desired concentration unit, average Blank signal (background) and average Negative Control signal (Concentration 0 : 100% viability).

Select how many concentration points you tested (2–12).

Input the concentration and average signal for each point.

Result: The tool calculates the exact IC50/EC50 value and reports if the potency is outside your tested range.

If you have any questions or confusions about the topic, ask Sophie AI!

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Solution Conversions Calculator: Molarity, Dilutions & Percentages

Whether you are making a molar stock from powder, preparing a percentage solution (w/v), or calculating a dilution series, this tool provides step-by-step recipes:

Select your calculation mode (e.g., Molarity, Dilution, or Dilution Factor).

Fill in the required fields (Molecular Weight, Target Volume, etc.).

Result: Receive a clear instruction, such as "Dissolve 5g in 80mL of water, then QS to 100mL," or "Mix 1 part sample with 9 parts buffer."

If you have any questions or confusions about the topic, ask Sophie AI!

Standard Curve Calculator – Linear Regression & Concentration Analysis

This tool generates a linear regression line (y = mx + b) from your standards and automatically calculates the concentration of your unknown samples, including Standard Deviation adjustments.

Enter your Concentration unit an dilution factor.

Select the number of Standards and Unknown Samples you have.

Enter the concentration and average signal (Data) for your standards to generate the curve. (Notice, first concentration Conc1 is 0 as your blank)

Enter the average data and the Standard Deviation (SD) for your samples.

Result: View a real-time scatter plot of your standard curve and a bar graph of your sample concentrations.

If you have any questions or confusions about the topic, ask Sophie AI!

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