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Stop guessing your CCK-8 incubation times! While the CCK-8 assay is the gold standard for cell viability due to its non-toxicity and high sensitivity, the "2-hour rule" often leads to poor data quality. This Ultimate Guide explains the science of WST-8 kinetics and provides a step-by-step pilot protocol to determine the perfect incubation time for your specific cell line. Learn to avoid saturation, handle suspension cells, and troubleshoot bubbles to get publication-ready res