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Unlock the full potential of your scratch assays! Are you confident in your cell migration results? This definitive guide reveals why positive and negative controls are non-negotiable for robust, publishable data. Learn to select the best controls—from EGF and serum to cytochalasin D—and integrate them seamlessly into your workflow. We provide step-by-step instructions and pro-tips to help you eliminate ambiguity, troubleshoot with precision, and make confident claims about y