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Tired of failed experiments due to poor cell confluency? The secret to perfect monolayers lies in one critical metric: cell doubling time. Stop guessing and start calculating! This definitive guide breaks down the simple four-step process to determine your cell line's precise growth rate. Learn the formula, apply it to your seeding strategy, and transform your cell culture workflow. Achieve reproducible results for every cytotoxicity, viability, and scratch assay.