Discover the Latest in Biomedical Research Innovation

Hunted through Prism's Analyze menu for ANCOVA? It isn't there, and GraphPad's own FAQ confirms they've never shipped one. Prism can still do it, through two documented routes most users never find: the simple-linear-regression slopes test for the parallel-slopes assumption, and multiple regression with dummy-coded groups for the adjusted comparison. Here are both step by step, plus an honest comparison with describing the same analysis in plain language instead.

Walk into most labs and you'll find a multi-seat GraphPad Prism license that only one or two people can actually use. Prism is powerful, but its learning curve takes months, so the license sits idle while everyone waits on the lab's 'stats person.' This piece makes the case for a different GraphPad Prism alternative: Sophie, the AI you just talk to. Describe your experiment in plain English and it picks the right test, runs it, and explains the result.

Are your p-values valid? The Shapiro-Wilk test is the "gold standard" for checking normality in biomedical research. This guide breaks down exactly how to perform the test in GraphPad Prism, SPSS, and R, how to interpret the results (p < 0.05 vs p > 0.05), and what to do when your data "fails" the test. Don't risk a rejected manuscript—master your statistical assumptions today.

Struggling with non-normal data in your research? The Mann-Whitney U test is the gold standard for comparing two independent groups when the t-test fails. In this guide, we break down the exact GraphPad Prism protocol, step-by-step. Learn how to enter data, configure the analysis for "Exact" p-values, and avoid the common reporting mistakes that annoy reviewers. Perfect for biomedical researchers needing a quick, accurate, and scientifically rigorous walkthrough.