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Staring at empty agar plates after a bacterial transformation is a frustrating experience for any researcher. But don't despair! This in-depth troubleshooting guide and FAQ will help you pinpoint the cause of your failed experiment. We'll explore common issues with competent cells, plasmid DNA, and your transformation protocol. With our expert tips and a systematic approach, you'll be on your way to a successful transformation and a plate full of colonies in no time.