Struggling with blank slides or patchy staining in IHC? The culprit is likely Antigen Retrieval. Whether it's a pH mismatch, microwave inconsistency, or over-fixation, this guide synthesizes expert protocols from CST, Thermo, and clinical researchers to fix your staining. Learn why switching from Citrate to Tris-EDTA could be the magic bullet and how to stop "exploding" your tissue.
