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Hit a wall with high back pressure during your protein purification? Don't toss that expensive column yet! This guide covers the immediate triage steps to save a clogged column and the essential "Clean Load" sample prep strategy (DNase, high-speed spins, and filtration) to prevent it from ever happening again. Master your FPLC workflow today.

Are inclusion bodies the bane of your existence or a hidden opportunity? For researchers working with recombinant proteins in E. coli, finding your target protein in an insoluble pellet is a common frustration. But science shows these aggregates are specific, reversible, and often highly pure. This article dives into the mechanisms of protein aggregation, offers proven strategies to prevent it, and provides a step-by-step guide to solubilizing and refolding your protein for m