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Yes, you can run an MTT assay on suspension cells, but don't dump the media! You risk losing your sample. Learn the two critical methods—Centrifugation vs. Direct Solubilization—to ensure accurate viability data without washing your cells down the drain.

Struggling to choose between MTT, WST-1, and CCK-8 for your cell viability experiments? This guide breaks down the critical differences in sensitivity, workflow, and toxicity. Discover why CCK-8 is replacing the "Gold Standard" MTT, how to avoid common interference errors with colored drugs, and which assay delivers the cleanest data for your specific cell lines.