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Struggling with inconsistent MTT assay results? The "standard" 5,000 cells/well might be ruining your data. This guide breaks down the exact "Standard Curve" protocol to find your optimal seeding density, ensuring linearity and reproducibility. Learn why metabolic state matters more than cell count and how to avoid the dreaded "Edge Effect."

Master the MTT assay with this comprehensive guide designed for accuracy and reproducibility. We break down the complex cell viability protocol into actionable steps, focusing on Primary Human Dermal Fibroblasts. Learn how to seed 10,000 cells/well, prepare 6 distinct product concentrations, and precisely measure metabolic activity using formazan detection at 540 nm.