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This definitive guide to Northern blotting synthesizes expert protocols for detecting miRNA, siRNA, and piRNA with unmatched sensitivity. We detail the critical steps, from high-resolution Urea-PAGE to the 50-fold sensitivity boost of EDC chemical cross-linking. Learn to master probe labeling (both 32P and non-radioactive biotin methods), optimize transfer, and troubleshoot common issues like multi-probe detection. This is your all-in-one resource.