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Dive into the world of gene expression analysis with our in-depth guide to Quantitative RT-PCR (qRT-PCR) protocols. Discover the power of this technique to unlock the secrets of RNA and learn the step-by-step process for obtaining accurate and reproducible results. From one-step vs. two-step assays to essential tips for experimental design and data analysis, this article is your ultimate resource for mastering qRT-PCR.