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Trizol extraction is the gold standard, but working with tissue requires mastering the critical homogenization step. This definitive guide summarizes expert protocols for success, from liquid nitrogen grinding to mechanical lysis. We detail the step-by-step Trizol protocol—phase separation, precipitation, and washing—and provide essential troubleshooting tips from real labs to help you avoid common pitfalls like salt contamination and poor solubility.

Dive into the world of gene expression analysis with our in-depth guide to Quantitative RT-PCR (qRT-PCR) protocols. Discover the power of this technique to unlock the secrets of RNA and learn the step-by-step process for obtaining accurate and reproducible results. From one-step vs. two-step assays to essential tips for experimental design and data analysis, this article is your ultimate resource for mastering qRT-PCR.