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Frustrated by peeling monolayers and mysterious gaps ruining your scratch assay? Don't discard that plate just yet! A single variable in your setup is the likely culprit, sabotaging your results before you even make the scratch. Uncover the simple cause behind monolayer detachment and learn the essential techniques to guarantee flawless, reproducible data in every single well. The definitive fix is inside.

Master a full range of ELISA & EIA techniques! This guide details Sandwich, Phosphorylation, and In-Cell ELISA protocols, plus EIA concepts, based on expert Sigma-Aldrich procedures. Learn step-by-step methods for accurate protein quantification and cellular analysis, from plate coating and cell fixation to signal detection. Elevate your research with tips for optimization, troubleshooting, and generating reproducible data.