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Genotyping PCR Shadow Bands: Diagnosis and Elimination
Struggling with messy PCR gels? "Shadow bands" and "stutter products" can ruin your genotyping data and waste days of lab work. Whether it's polymerase slippage in microsatellites or simple contamination in your master mix, the fix is often simpler than you think. From switching to "Proofreading" enzyme blends to the "Low-Temp Denaturation" trick, we’ve compiled the ultimate scientific guide to cleaning up your PCR. Stop guessing and start getting publication-quality bands to
2 days ago4 min read
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