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The Hematoxylin and Eosin (H&E) stain is the gold standard for pathologists, but mastering it requires precision. Our comprehensive guide synthesizes expert protocols to break down the complete H&E staining procedure, from deparaffinization to mounting. We detail the "why" behind the stain and provide key safety SOPs and troubleshooting tips to help you achieve crisp blue nuclei and vibrant pink cytoplasm for publication-quality results.