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Are inclusion bodies the bane of your existence or a hidden opportunity? For researchers working with recombinant proteins in E. coli, finding your target protein in an insoluble pellet is a common frustration. But science shows these aggregates are specific, reversible, and often highly pure. This article dives into the mechanisms of protein aggregation, offers proven strategies to prevent it, and provides a step-by-step guide to solubilizing and refolding your protein for m

Struggling with faint or invisible bands in your Western blots? This in-depth guide provides a detailed, step-by-step protocol for detecting low-abundance proteins. We cover critical optimizations from strategic sample preparation and lysate concentration to advanced detection using high-sensitivity ECL substrates. Learn how to choose the right buffers, membranes, and antibodies, and master techniques for enhancing your signal-to-noise ratio.