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Master a fundamental lab technique! Passaging adherent cells is essential for healthy, continuous cell cultures, but it's a process with many critical steps. Our comprehensive protocol, synthesized from 8 expert sources, guides you through every stage. Learn the 'why' behind washing with PBS, the right way to trypsinize and neutralize, and the calculations for perfect seeding density. Move beyond basic subculturing and ensure the viability and success of your cell lines with