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Unlock the power of crystal violet staining! This comprehensive, step-by-step Standard Operating Procedure (SOP) demystifies the widely-used technique for quantifying adherent cell numbers. From cell seeding and fixation to precise staining, washing, and solubilization, each phase is detailed for accuracy. Learn to troubleshoot common issues and ensure reliable, reproducible results for your cell viability, proliferation, or cytotoxicity assays.