Discover the Latest in Biomedical Research Innovation

Master the MTT assay with this comprehensive guide designed for accuracy and reproducibility. We break down the complex cell viability protocol into actionable steps, focusing on Primary Human Dermal Fibroblasts. Learn how to seed 10,000 cells/well, prepare 6 distinct product concentrations, and precisely measure metabolic activity using formazan detection at 540 nm.

Unlock the power of your cell viability data! This guide demystifies the two most critical metrics in dose-response analysis: IC 50 ​ and EC 50 ​ . Discover the key difference between measuring inhibition (IC 50 ​ ) and activation (EC 50 ​ ), learn the step-by-step process for calculating these values, and see how they reveal the synergistic power of combination therapies.