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Struggling with faint or invisible bands in your Western blots? This in-depth guide provides a detailed, step-by-step protocol for detecting low-abundance proteins. We cover critical optimizations from strategic sample preparation and lysate concentration to advanced detection using high-sensitivity ECL substrates. Learn how to choose the right buffers, membranes, and antibodies, and master techniques for enhancing your signal-to-noise ratio.

Supercharge your protein analysis! Explore Western Blotting protocols in-depth. This guide details everything from sample prep and SDS-PAGE to specific transfer methods (Tank vs. Semi-Dry) and detection options (Chemiluminescent, Fluorescent, Colorimetric). Learn how to choose the right method, optimise steps, and accurately detect and quantify your proteins. Get the reliable, high-quality Western Blot data you need for your research with these step-by-step instructions and k