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Master a fundamental lab technique! Passaging adherent cells is essential for healthy, continuous cell cultures, but it's a process with many critical steps. Our comprehensive protocol, synthesized from 8 expert sources, guides you through every stage. Learn the 'why' behind washing with PBS, the right way to trypsinize and neutralize, and the calculations for perfect seeding density. Move beyond basic subculturing and ensure the viability and success of your cell lines with

Mycoplasma contamination is the silent killer of research. This invisible threat doesn't make your media cloudy, but it systematically destroys the validity of your experiments by altering cell metabolism and gene expression. How do you fight an enemy you can't see? This article provides a complete, SEO-optimized protocol for Mycoplasma testing in cell culture. We dive into the "why" and "how" of detection, comparing the gold standard culture method with rapid PCR and visual