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Stop guessing your cell seeding densities! Consistent experiments start with accurate cell growth kinetics. This guide breaks down the "Cell Doubling Time" formula, explains why it matters for reproducibility, and provides a step-by-step protocol for measuring it. We also introduce the CLYTE Cell Doubling Calculator—a free tool that automates the math so you can hit perfect confluency every time. Master your culture conditions and eliminate experimental drift today.

Master a fundamental lab technique! Passaging adherent cells is essential for healthy, continuous cell cultures, but it's a process with many critical steps. Our comprehensive protocol, synthesized from 8 expert sources, guides you through every stage. Learn the 'why' behind washing with PBS, the right way to trypsinize and neutralize, and the calculations for perfect seeding density. Move beyond basic subculturing and ensure the viability and success of your cell lines with