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Struggling to choose between MTT, WST-1, and CCK-8 for your cell viability experiments? This guide breaks down the critical differences in sensitivity, workflow, and toxicity. Discover why CCK-8 is replacing the "Gold Standard" MTT, how to avoid common interference errors with colored drugs, and which assay delivers the cleanest data for your specific cell lines.

High background absorbance in MTT assays can ruin weeks of research, often caused by chemical interference, serum precipitation, or microbial contamination. This guide dives into the "purple haze" of false signals, offering expert troubleshooting tips—from using proper background controls to swapping media—to ensure your OD readings reflect true cellular health. Discover how to refine your protocol and when to switch to alternative assays.