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MTT Assay vs. Live/Dead Staining: Your Guide to The Right Cell Viability Assay
Are you struggling to choose between the cost-effective MTT assay and the visually striking Live/Dead fluorescence staining? This guide breaks down the science, mechanisms, and pros and cons of the two most popular cell viability methods. Whether you are running high-throughput drug screenings or analyzing complex 3D spheroids, understanding the difference between metabolic activity and membrane integrity is key. Dive in to discover which assay reigns supreme for your specifi
Dec 22, 20255 min read


Guide to Calculating and Interpreting IC50 & EC50 Values in Cell Viability Assays
Unlock the power of your cell viability data! This guide demystifies the two most critical metrics in dose-response analysis: IC
50
and EC
50
. Discover the key difference between measuring inhibition (IC
50
) and activation (EC
50
), learn the step-by-step process for calculating these values, and see how they reveal the synergistic power of combination therapies.
Sep 17, 20255 min read
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