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Struggling with inconsistent MTT assay results? The "standard" 5,000 cells/well might be ruining your data. This guide breaks down the exact "Standard Curve" protocol to find your optimal seeding density, ensuring linearity and reproducibility. Learn why metabolic state matters more than cell count and how to avoid the dreaded "Edge Effect."

High background absorbance in MTT assays can ruin weeks of research, often caused by chemical interference, serum precipitation, or microbial contamination. This guide dives into the "purple haze" of false signals, offering expert troubleshooting tips—from using proper background controls to swapping media—to ensure your OD readings reflect true cellular health. Discover how to refine your protocol and when to switch to alternative assays.

Master the MTT assay with this comprehensive guide designed for accuracy and reproducibility. We break down the complex cell viability protocol into actionable steps, focusing on Primary Human Dermal Fibroblasts. Learn how to seed 10,000 cells/well, prepare 6 distinct product concentrations, and precisely measure metabolic activity using formazan detection at 540 nm.