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Master Crystal Violet Staining: A Step-by-Step Protocol for Accurate Cell Quantification
Unlock the power of crystal violet staining! This comprehensive, step-by-step Standard Operating Procedure (SOP) demystifies the widely-used technique for quantifying adherent cell numbers. From cell seeding and fixation to precise staining, washing, and solubilization, each phase is detailed for accuracy. Learn to troubleshoot common issues and ensure reliable, reproducible results for your cell viability, proliferation, or cytotoxicity assays.
Jun 187 min read
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How to use ImageJ for Scratch Assay Analysis: A Powerful Tool for Cell Migration Measurement
Learn how to measure cell migration in wound healing assays! This new ImageJ plugin offers unparalleled accuracy and efficiency, overcoming the tediousness of manual methods and the unreliability of some automated systems. By providing a semi-automated, user-friendly interface, and a clear step-by-step process, it empowers researchers in cancer biology, tissue regeneration, and drug discovery to obtain more consistent and reproducible results, faster. Free, accessible, and po
Jun 165 min read
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How to do Wound Healing (Scratch) Assay for Cell Migration Analysis
Perform the wound healing assay with this detailed step-by-step protocol. Learn the materials, methods, and procedures for seeding cells, creating a consistent scratch, imaging cell migration over time using microscopy, and analyzing wound closure rates. Includes tips for optimization and controlling proliferation. Essential technique for studying in vitro cell motility, cancer research, and drug screening. This guide provides the technical details needed for reproducible res
May 56 min read
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Unlocking Cell Secrets: A Deep Dive into the In Vitro Scratch Assay for Cell Migration Analysis
Unlock cell movement secrets! Discover the in vitro scratch assay, a key, low-cost method to study cell migration. Learn how this technique mimics wound healing and analyzes cell-cell/cell-matrix interactions using basic lab tools & microscopy. We cover its power, versatility with transfected cells, and crucial considerations like standardization for understanding cell behavior in vitro. Essential reading for cell biology insights!
Apr 212 min read
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