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Are inclusion bodies the bane of your existence or a hidden opportunity? For researchers working with recombinant proteins in E. coli, finding your target protein in an insoluble pellet is a common frustration. But science shows these aggregates are specific, reversible, and often highly pure. This article dives into the mechanisms of protein aggregation, offers proven strategies to prevent it, and provides a step-by-step guide to solubilizing and refolding your protein for m

This frustrating (and common) problem can ruin days of work, but the fix is often simple. Our comprehensive troubleshooting guide diagnoses the real reason for your patchy bands. We cover everything from the #1 culprit—air bubbles in your transfer sandwich—to aggregated antibodies, uneven gel polymerization, and improper sample prep. Stop guessing and start fixing. This article provides clear, actionable solutions to rescue your blot and get the clean, sharp bands you need.