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Struggling with blank slides or patchy staining in IHC? The culprit is likely Antigen Retrieval. Whether it's a pH mismatch, microwave inconsistency, or over-fixation, this guide synthesizes expert protocols from CST, Thermo, and clinical researchers to fix your staining. Learn why switching from Citrate to Tris-EDTA could be the magic bullet and how to stop "exploding" your tissue.

Stop the frustration of watching your precious tissue sections float away during an IHC run. Losing samples is one of the most common and costly failures in a histology lab, but it is almost entirely avoidable. It's not just "bad slides"—your entire protocol, from fixation and processing to your drying time and antigen retrieval technique, plays a crucial role. Learn why a 20-minute bake isn't enough, high-pH buffers problems, and the tricks to ensure your tissue stays firmly