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Mastering Subculture: A Detailed Protocol for Passaging Adherent Cells
Master a fundamental lab technique! Passaging adherent cells is essential for healthy, continuous cell cultures, but it's a process with many critical steps. Our comprehensive protocol, synthesized from 8 expert sources, guides you through every stage. Learn the 'why' behind washing with PBS, the right way to trypsinize and neutralize, and the calculations for perfect seeding density. Move beyond basic subculturing and ensure the viability and success of your cell lines with
Nov 214 min read
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Guide to Mycoplasma-Free Cultures: Mycoplasma Testing Protocol
Mycoplasma contamination is the silent killer of research. This invisible threat doesn't make your media cloudy, but it systematically destroys the validity of your experiments by altering cell metabolism and gene expression. How do you fight an enemy you can't see? This article provides a complete, SEO-optimized protocol for Mycoplasma testing in cell culture. We dive into the "why" and "how" of detection, comparing the gold standard culture method with rapid PCR and visual
Nov 148 min read
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Break the Ice: How to Thaw Frozen Cells for Optimal Viability
Unlock the full potential of your cryopreserved cells with our definitive guide to thawing. From the crucial first moments in the water bath to the final plating, we'll walk you through the essential steps and best practices to ensure maximum cell viability and a successful start to your experiments. Learn the secrets to reviving your frozen cells and set your research up for success.
Oct 34 min read
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