Discover the Latest in Biomedical Research Innovation

Hunted through Prism's Analyze menu for ANCOVA? It isn't there, and GraphPad's own FAQ confirms they've never shipped one. Prism can still do it, through two documented routes most users never find: the simple-linear-regression slopes test for the parallel-slopes assumption, and multiple regression with dummy-coded groups for the adjusted comparison. Here are both step by step, plus an honest comparison with describing the same analysis in plain language instead.

A no-fluff, step-by-step scratch assay imaging protocol for images your software can actually measure. Twenty-six numbered steps take you from pixel calibration and Köhler alignment, through Time 0 capture with locked exposure, to returning to identical fields at later time points. Includes an equipment table, an acceptance-criteria checklist to run before you analyze, and a troubleshooting table mapping every common symptom to the step that fixes it.

When medical residents were surveyed on interpreting a p-value, 88% were confident they understood it and 100% got it wrong. That gap is why p-value mistakes survive peer review: nobody thinks they're making one. This piece covers the five most common errors in biomedical papers, several measured at scale in real journals: the inverse probability fallacy, the replication fallacy, 'trending toward significance', p-hacking, and treating 0.05 as a bright line.

A no-fluff, step-by-step cell imaging protocol for microscopy images you can actually quantify. Eighteen numbered steps take you from Köhler setup and pixel calibration, through setting exposure on your brightest sample so nothing saturates, to acquiring the whole set under locked, identical settings. Works for brightfield, phase contrast, and fluorescence. Includes an equipment table, an acceptance-criteria checklist, and a troubleshooting table mapping each symptom to the s

Your p-value can confirm an effect is real and say nothing about whether it's big enough to matter. Effect size answers that second question, and for a two-group comparison Cohen's d is the standard measure: the mean difference divided by the pooled standard deviation. This guide covers how to calculate and report Cohen's d, why to report it alongside every p-value and confidence interval, and how to interpret it without leaning on the 0.2/0.5/0.8 benchmarks that often don't

Unlock the power of your clinical data with the Box and Whisker Plot! Learn the definitive step-by-step method for calculating the five-number summary—Min, Q1, Median, Q3, Max—and visualizing data distribution. From identifying critical outliers using the 1.5 x IQR rule to comparing drug efficacy in biomedical research, this guide is your key to superior data visualization that ranks and informs. Stop relying on means alone. Start seeing your data's true spread!

Running 10,000 gene expression tests? You might get 500 false positives by random chance. This is the "multiple hypothesis testing problem," and it's a critical flaw in modern biomedical research. The traditional fix, the Bonferroni correction, is so strict it often causing you to miss real discoveries. But a more powerful, modern method—the False Discovery Rate (FDR)—offers a revolutionary trade-off: what if you could control the proportion of false positives, not just the c