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Deep Dive into the Transwell Migration and Invasion Assay
Discover the power of the Transwell migration and invasion assay, a fundamental tool in cell biology. This article delves into the principles, protocols, and applications of this versatile technique, explaining how it allows scientists to study cell movement in detail. Whether you're a seasoned researcher or new to the field, our comprehensive guide will provide you with the knowledge you need to master the Transwell assay and unlock the secrets of cell migration and invasion
Oct 68 min read
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Guide to Calculating and Interpreting IC50 & EC50 Values in Cell Viability Assays
Unlock the power of your cell viability data! This guide demystifies the two most critical metrics in dose-response analysis: IC
50
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and EC
50
​
. Discover the key difference between measuring inhibition (IC
50
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) and activation (EC
50
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), learn the step-by-step process for calculating these values, and see how they reveal the synergistic power of combination therapies.
Sep 175 min read
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Scratch Assay Migration vs Proliferation: Is Your Wound Closure From Cell Proliferation Rather Than Migration?
Struggling to distinguish between cell migration and proliferation in your scratch assays? Uncover the definitive solution with Mitomycin C! This powerful DNA crosslinking agent halts cell division, allowing you to isolate and measure true cell motility with confidence. Our comprehensive guide provides a step-by-step protocol, from preparing your stock solution to analyzing your results, ensuring your wound healing data is both accurate and reproducible.
Jul 115 min read
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