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2026 Comparative Guide to Tools for Scratch Assay Cell Migration Analysis
Struggling with manual scratch assay analysis? This 2026 guide compares 7 leading image analysis tools, including Soφ AI, ImageJ, and CellProfiler. We break down the pros, cons, and best use cases for each—from high-throughput AI automation to single-cell tracking. Whether you need speed, standardization, or total customization, discover which platform fits your specific research needs.
19 hours ago4 min read


Mastering Subculture: A Detailed Protocol for Passaging Adherent Cells
Master a fundamental lab technique! Passaging adherent cells is essential for healthy, continuous cell cultures, but it's a process with many critical steps. Our comprehensive protocol, synthesized from 8 expert sources, guides you through every stage. Learn the 'why' behind washing with PBS, the right way to trypsinize and neutralize, and the calculations for perfect seeding density. Move beyond basic subculturing and ensure the viability and success of your cell lines with
Nov 21, 20254 min read


10 Reasons Your Cells Won't Attach to the Flask : Low Adhesion and How to Fix It!
Are your cells refusing to stick to the flask? You're not alone. This frustrating problem can derail your experiments and leave you scratching your head. But fear not! Our in-depth guide explores the top 10 reasons for poor cell attachment, from problems with your culture vessel to issues with cell health, media, and more. We'll help you troubleshoot the adhesion problem and get your cells back to doing what they do best: growing and dividing.
Oct 15, 20257 min read


Break the Ice: How to Thaw Frozen Cells for Optimal Viability
Unlock the full potential of your cryopreserved cells with our definitive guide to thawing. From the crucial first moments in the water bath to the final plating, we'll walk you through the essential steps and best practices to ensure maximum cell viability and a successful start to your experiments. Learn the secrets to reviving your frozen cells and set your research up for success.
Oct 3, 20254 min read


Cell Doubling Time Formula: Your Ultimate Guide to Perfect Confluency
Tired of failed experiments due to poor cell confluency? The secret to perfect monolayers lies in one critical metric: cell doubling time. Stop guessing and start calculating! This definitive guide breaks down the simple four-step process to determine your cell line's precise growth rate. Learn the formula, apply it to your seeding strategy, and transform your cell culture workflow. Achieve reproducible results for every cytotoxicity, viability, and scratch assay.
Sep 22, 20254 min read


Is Overconfluency Ruining Your Wound Healing (Scratch) Assay? How to Fix Peeling Monolayers
Frustrated by peeling monolayers and mysterious gaps ruining your scratch assay? Don't discard that plate just yet! A single variable in your setup is the likely culprit, sabotaging your results before you even make the scratch.
Uncover the simple cause behind monolayer detachment and learn the essential techniques to guarantee flawless, reproducible data in every single well. The definitive fix is inside.
Jul 18, 20254 min read
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