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Guide to Calculating and Interpreting IC50 & EC50 Values in Cell Viability Assays
Unlock the power of your cell viability data! This guide demystifies the two most critical metrics in dose-response analysis: IC
50
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and EC
50
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. Discover the key difference between measuring inhibition (IC
50
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) and activation (EC
50
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), learn the step-by-step process for calculating these values, and see how they reveal the synergistic power of combination therapies.
Sep 175 min read
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Beyond the Image: Your Ultimate Guide to Statistical Analysis of Wound Healing Assays
Your scratch assay images look amazing, but are they statistically significant? Don't let your groundbreaking research get rejected for weak data analysis. Many researchers see a clear difference in cell migration but struggle to prove it with the right stats. This guide breaks down the intimidating process into simple, actionable steps. Learn how to choose the right test (t-test vs. ANOVA), avoid common pitfalls like pseudo-replication, and confidently turn your results into
Jul 215 min read
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Scratch Assay Migration vs Proliferation: Is Your Wound Closure From Cell Proliferation Rather Than Migration?
Struggling to distinguish between cell migration and proliferation in your scratch assays? Uncover the definitive solution with Mitomycin C! This powerful DNA crosslinking agent halts cell division, allowing you to isolate and measure true cell motility with confidence. Our comprehensive guide provides a step-by-step protocol, from preparing your stock solution to analyzing your results, ensuring your wound healing data is both accurate and reproducible.
Jul 115 min read
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