Discover the Latest in Biomedical Research Innovation

Are you planning a pre-clinical study but struggling with the statistics? Determining the correct sample size is the single most critical step to ensure your biomedical research is ethical, economic, and publishable. In this detailed guide, we break down how to use G*Power, the industry-standard free software. From understanding Alpha and Beta errors to a step-by-step tutorial on t-tests for drug efficacy, we provide everything you need to justify your sample size in your nex

2025 was the year CLYTE evolved from a hardware startup into a biomedical platform. From validating the CytCut device to launching Sophie AI, we achieved ~50k monthly visitors and 5 million impressions with extreme capital efficiency. Discover how we uncovered a massive market gap in lab protocols and secured our place in the future of lab automation.

From antioxidant vitamins and plant flavonoids that mimic cell activity to porphyrins that destroy the signal, many common compounds can skew your data, leading to false positives or negatives. This article explores the specific chemicals that disrupt tetrazolium assays, explains the mechanisms behind these artifacts, and provides essential best practices to ensure your cytotoxicity data is accurate and reproducible.

Discover how CLYTE is transforming biomedical research by aligning with the UN Sustainable Development Goals. From replacing disposable pipette tips with the reusable CytCut device (SDG 12) to democratizing access to the Soφ AI assistant (SDG 4), CLYTE is building a smarter, more sustainable future for science. Read how their specific innovations are solving the reproducibility crisis and driving global health progress.

Stop wasting time and money on endless minipreps! Colony PCR is the ultimate lab hack for molecular cloning, allowing you to rapidly screen bacterial or yeast colonies for your desired DNA insert in just a few hours. This definitive guide cuts through the noise and provides a detailed, step-by-step protocol for success. We dive deep into the one step most scientists get wrong—cell lysis and reveal primer design strategies!

The Hematoxylin and Eosin (H&E) stain is the gold standard for pathologists, but mastering it requires precision. Our comprehensive guide synthesizes expert protocols to break down the complete H&E staining procedure, from deparaffinization to mounting. We detail the "why" behind the stain and provide key safety SOPs and troubleshooting tips to help you achieve crisp blue nuclei and vibrant pink cytoplasm for publication-quality results.

Unlock the power of your biomedical data! Dose-response curves are vital, but non-linear relationships demand sophisticated analysis. This guide demystifies non-linear regression using GraphPad Prism. Learn to choose the right model, understand key parameters, set crucial constraints, and interpret results like confidence intervals and R². We cover data prep, global fitting for complex scenarios, and troubleshooting common issues like ambiguous fits. Master Prism for accurate

Ready to master apoptosis detection? This comprehensive guide delivers a detailed, step-by-step TUNEL assay protocol, the gold standard for visualizing DNA fragmentation. We've synthesized expert advice from top scientific sources to cover the core principle, a complete protocol for cells and tissues, and the critical troubleshooting tips you need to avoid false positives. Learn how to get reliable, publication-ready data by optimizing fixation, permeabilization, and controls

Unlock the secrets of the most essential technique in molecular biology! This comprehensive guide to the plasmid miniprep protocol takes you from a single bacterial colony to pure, high-yield DNA. We dive deep into the "3-buffer magic" of alkaline lysis and provide a detailed, step-by-step protocol for both modern silica spin-columns and traditional precipitation methods. Learn to troubleshoot common problems like low yield and gDNA contamination, and get your DNA ready for a

Trizol extraction is the gold standard, but working with tissue requires mastering the critical homogenization step. This definitive guide summarizes expert protocols for success, from liquid nitrogen grinding to mechanical lysis. We detail the step-by-step Trizol protocol—phase separation, precipitation, and washing—and provide essential troubleshooting tips from real labs to help you avoid common pitfalls like salt contamination and poor solubility.

Seeing an unexpected band in your PCR negative control (NTC) is a frustrating problem that invalidates your results. But what does it mean? This article dives deep into the two most likely culprits: DNA contamination and primer-dimers. Explore contamination from reagents, equipment, or even the air can introduce unwanted DNA, and why your primers might be binding to each other. Most importantly, we provide a step-by-step action plan to decontaminate your workspace and get cle

This definitive guide to Northern blotting synthesizes expert protocols for detecting miRNA, siRNA, and piRNA with unmatched sensitivity. We detail the critical steps, from high-resolution Urea-PAGE to the 50-fold sensitivity boost of EDC chemical cross-linking. Learn to master probe labeling (both 32P and non-radioactive biotin methods), optimize transfer, and troubleshoot common issues like multi-probe detection. This is your all-in-one resource.

Unlock the power of genetic engineering with the heat shock transformation method! Learn the precise science behind creating chemically competent cells using calcium chloride and the critical 42 C temperature pulse that forces bacteria to take up life-changing plasmid DNA. Master the detailed protocol steps—from chilling on ice to the crucial outgrowth phase—to achieve maximum transformation efficiency in your molecular cloning experiments and revolutionize protein production

Are your cells refusing to stick to the flask? You're not alone. This frustrating problem can derail your experiments and leave you scratching your head. But fear not! Our in-depth guide explores the top 10 reasons for poor cell attachment, from problems with your culture vessel to issues with cell health, media, and more. We'll help you troubleshoot the adhesion problem and get your cells back to doing what they do best: growing and dividing.

Unlock the full potential of your cryopreserved cells with our definitive guide to thawing. From the crucial first moments in the water bath to the final plating, we'll walk you through the essential steps and best practices to ensure maximum cell viability and a successful start to your experiments. Learn the secrets to reviving your frozen cells and set your research up for success.

Dive into the world of gene expression analysis with our in-depth guide to Quantitative RT-PCR (qRT-PCR) protocols. Discover the power of this technique to unlock the secrets of RNA and learn the step-by-step process for obtaining accurate and reproducible results. From one-step vs. two-step assays to essential tips for experimental design and data analysis, this article is your ultimate resource for mastering qRT-PCR.

CLYTE, a trailblazing AI-biotech startup, is revolutionizing biomedical research. With groundbreaking innovations like the AI lab assistant Soφ, the precision tool CellCut, and the upcoming cell culture monitor Cadmus, CLYTE is tackling inefficiencies and enhancing research standardization. Their unique "First-Client Initiative" offers researchers free access to beta products, fostering a collaborative approach to scientific discovery and accelerating the pace of healthcare i

Struggling with faint or invisible bands in your Western blots? This in-depth guide provides a detailed, step-by-step protocol for detecting low-abundance proteins. We cover critical optimizations from strategic sample preparation and lysate concentration to advanced detection using high-sensitivity ECL substrates. Learn how to choose the right buffers, membranes, and antibodies, and master techniques for enhancing your signal-to-noise ratio.

Unlock the secrets of cellular communication! Our step-by-step guide to the Proximity Ligation Assay (PLA) provides a comprehensive walkthrough of this powerful technique. From sample preparation to data analysis, we'll show you how to visualize protein-protein interactions with stunning clarity. Whether you're a seasoned researcher or new to the field, this guide will equip you with the knowledge and confidence to perform successful PLA experiments.

Struggling with tedious lab protocols and failed experiments? As a biomedical researcher, I was too, until I discovered Soφ AI. This isn't just another AI tool; it's a revolutionary lab assistant that has transformed my research. From generating customized protocols in minutes to troubleshooting experiments and even generating novel hypotheses, Soφ AI is the partner every researcher dreams of. Read my personal review to see how this game-changing AI can supercharge your resea