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Guide to Calculating and Interpreting IC50 & EC50 Values in Cell Viability Assays
Unlock the power of your cell viability data! This guide demystifies the two most critical metrics in dose-response analysis: IC
50
​
and EC
50
​
. Discover the key difference between measuring inhibition (IC
50
​
) and activation (EC
50
​
), learn the step-by-step process for calculating these values, and see how they reveal the synergistic power of combination therapies.
Sep 175 min read
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The Coolest Assay to Learn! Step-by-Step Guide to the Checkerboard Assay
Struggling to prove if two compounds are truly better together? Stop guessing and start quantifying. The Checkerboard Assay is the gold-standard method for determining if your products exhibit synergy, additivity, or antagonism. This comprehensive guide provides a detailed, step-by-step protocol for setting up the assay, from cell seeding to data analysis using the Bliss Independence Model.
Sep 156 min read
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