Revolutionizing In Vitro Scratch Assays: How CytCut is Setting a New Standard in Cell Migration Research

1.0: CytCut 3.0 (Formerly Cellcut)

Cell migration plays a vital role in numerous biological processes, including wound healing, cancer metastasis, and tissue regeneration. To study these processes in vitro, researchers have long relied on the 2D scratch assay—also known as the wound healing assay—a simple yet powerful technique to observe and quantify cell migration.

But here's the problem: traditional scratch assays, often performed using pipette tips, are inconsistent, time-consuming, and subject to human variability. This is where CytCut by CLYTE comes in—a high-throughput, precision-engineered device designed to deliver reproducible scratch assays across multi-well plates in seconds.

What Is CytCut?

CytCut is a wounding tool that creates uniform scratches in 48-well plates with a single, smooth motion. Built for repeatability and speed, it eliminates the inconsistency of manual scratching and drastically improves experimental reproducibility in cell migration studies.

Why Traditional Scratch Assays Fall Short

If you’ve ever tried using a pipette tip or a needle to scratch your wells, you know the frustration:

• Scratch width and depth are inconsistent

• Cell detachment is often uneven

• Time-consuming when applied to large sample sizes

• Poor comparability across experiments or between labs

These issues not only compromise data quality but can also undermine the validity of your results.

CytCut Solves All of That

With CytCut, you can:

✅ Perform simultaneous scratches across entire rows or columns

✅ Generate consistent wound areas every time

✅ Increase throughput by 5–10×

✅ Reduce experimental variability

✅ Easily sterilize and reuse the device

Designed for High-Impact Cell Migration Research

Whether you’re investigating:

• Cancer cell migration

• Drug response testing

• Tissue engineering

• Wound healing

… CytCut ensures you get accurate, reproducible data with minimal hassle.

Its modular design includes a Plate holder-scratch guide, and a simple spiked top section, all optimized for ease of use, sterilization, and longevity.

Backed by Alpha & Beta Testing

Early adopters in academic and industrial labs reported positive feedback on:

• Increased speed and throughput

• Reliability and usability

• Compatibility with imaging systems

You can read more in our alpha testing review.

It's important to note that the product is still at beta stage and the output is not perfect; however through the beta testing and our FCI program, we are improving the product based on researchers feedback and desires, to create a gold standard in cell migration assays.

Join the First-Client Initiative

Through our First-Client Initiative, selected research partners can receive Up to one year of free access to CytCut in exchange for feedback and impact data. This is your chance to try a revolutionary new tool without the high costs—just great science.