Discover the Latest in Biomedical Research Innovation

High background absorbance in MTT assays can ruin weeks of research, often caused by chemical interference, serum precipitation, or microbial contamination. This guide dives into the "purple haze" of false signals, offering expert troubleshooting tips—from using proper background controls to swapping media—to ensure your OD readings reflect true cellular health. Discover how to refine your protocol and when to switch to alternative assays.

Stop wasting hours on manual lab math! Discover the ultimate guide to CLYTE’s free Biomedical Research Calculators. From automating cell seeding recipes and calculating IC50/EC50 values to generating linear regression standard curves, this suite streamlines your workflow. Learn how to eliminate calculation errors and boost experimental reproducibility today.

Is a RIN score below 7 the end of the road for your sequencing project? Not necessarily. While the "Gold Standard" of RIN > 8 is ideal for Illumina PolyA sequencing, modern techniques have rewritten the rules. From microRNA stability to Ribodepletion strategies for ancient samples, this article explores how to salvage data from degraded RNA. Learn why the RIN 7 cutoff is a myth for certain applications and discover the top 10 ways to improve your RNA isolation before you even

2025 was the year CLYTE evolved from a hardware startup into a biomedical platform. From validating the CytCut device to launching Sophie AI, we achieved ~50k monthly visitors and 5 million impressions with extreme capital efficiency. Discover how we uncovered a massive market gap in lab protocols and secured our place in the future of lab automation.

From antioxidant vitamins and plant flavonoids that mimic cell activity to porphyrins that destroy the signal, many common compounds can skew your data, leading to false positives or negatives. This article explores the specific chemicals that disrupt tetrazolium assays, explains the mechanisms behind these artifacts, and provides essential best practices to ensure your cytotoxicity data is accurate and reproducible.

Are inclusion bodies the bane of your existence or a hidden opportunity? For researchers working with recombinant proteins in E. coli, finding your target protein in an insoluble pellet is a common frustration. But science shows these aggregates are specific, reversible, and often highly pure. This article dives into the mechanisms of protein aggregation, offers proven strategies to prevent it, and provides a step-by-step guide to solubilizing and refolding your protein for m

Scientific research has a plastic problem. Every year, laboratories perform an estimated 3 million "scratch assays" using disposable pipette tips, generating around 200 tons of contaminated plastic waste that cannot be recycled. CytCut offers a solution: a reusable device that eliminates this waste at the source. By replacing single-use tips and improving experimental reproducibility, CytCut cuts carbon footprints by up to 11-fold, proving that science can be rigorous without

Master the MTT assay with this comprehensive guide designed for accuracy and reproducibility. We break down the complex cell viability protocol into actionable steps, focusing on Primary Human Dermal Fibroblasts. Learn how to seed 10,000 cells/well, prepare 6 distinct product concentrations, and precisely measure metabolic activity using formazan detection at 540 nm.