Discover the Latest in Biomedical Research Innovation

Stop guessing your cell seeding densities! Consistent experiments start with accurate cell growth kinetics. This guide breaks down the "Cell Doubling Time" formula, explains why it matters for reproducibility, and provides a step-by-step protocol for measuring it. We also introduce the CLYTE Cell Doubling Calculator—a free tool that automates the math so you can hit perfect confluency every time. Master your culture conditions and eliminate experimental drift today.

High background absorbance in MTT assays can ruin weeks of research, often caused by chemical interference, serum precipitation, or microbial contamination. This guide dives into the "purple haze" of false signals, offering expert troubleshooting tips—from using proper background controls to swapping media—to ensure your OD readings reflect true cellular health. Discover how to refine your protocol and when to switch to alternative assays.

From antioxidant vitamins and plant flavonoids that mimic cell activity to porphyrins that destroy the signal, many common compounds can skew your data, leading to false positives or negatives. This article explores the specific chemicals that disrupt tetrazolium assays, explains the mechanisms behind these artifacts, and provides essential best practices to ensure your cytotoxicity data is accurate and reproducible.

Master a fundamental lab technique! Passaging adherent cells is essential for healthy, continuous cell cultures, but it's a process with many critical steps. Our comprehensive protocol, synthesized from 8 expert sources, guides you through every stage. Learn the 'why' behind washing with PBS, the right way to trypsinize and neutralize, and the calculations for perfect seeding density. Move beyond basic subculturing and ensure the viability and success of your cell lines with

Are your cells refusing to stick to the flask? You're not alone. This frustrating problem can derail your experiments and leave you scratching your head. But fear not! Our in-depth guide explores the top 10 reasons for poor cell attachment, from problems with your culture vessel to issues with cell health, media, and more. We'll help you troubleshoot the adhesion problem and get your cells back to doing what they do best: growing and dividing.

Unlock the full potential of your cryopreserved cells with our definitive guide to thawing. From the crucial first moments in the water bath to the final plating, we'll walk you through the essential steps and best practices to ensure maximum cell viability and a successful start to your experiments. Learn the secrets to reviving your frozen cells and set your research up for success.