Discover the Latest in Biomedical Research Innovation

Stop drowning in data and start building a case. Most preclinical failures aren't technical—they're architectural. Discover why "Evidence Architecture" is the missing framework your biotech needs to survive FDA scrutiny, and hear about the rumored AI breakthrough from CLYTE that could change the game forever.

2025 was the year CLYTE evolved from a hardware startup into a biomedical platform. From validating the CytCut device to launching Sophie AI, we achieved ~50k monthly visitors and 5 million impressions with extreme capital efficiency. Discover how we uncovered a massive market gap in lab protocols and secured our place in the future of lab automation.

From antioxidant vitamins and plant flavonoids that mimic cell activity to porphyrins that destroy the signal, many common compounds can skew your data, leading to false positives or negatives. This article explores the specific chemicals that disrupt tetrazolium assays, explains the mechanisms behind these artifacts, and provides essential best practices to ensure your cytotoxicity data is accurate and reproducible.

Master the MTT assay with this comprehensive guide designed for accuracy and reproducibility. We break down the complex cell viability protocol into actionable steps, focusing on Primary Human Dermal Fibroblasts. Learn how to seed 10,000 cells/well, prepare 6 distinct product concentrations, and precisely measure metabolic activity using formazan detection at 540 nm.

Are you struggling to choose between the cost-effective MTT assay and the visually striking Live/Dead fluorescence staining? This guide breaks down the science, mechanisms, and pros and cons of the two most popular cell viability methods. Whether you are running high-throughput drug screenings or analyzing complex 3D spheroids, understanding the difference between metabolic activity and membrane integrity is key. Dive in to discover which assay reigns supreme for your specifi

Stop wasting time and money on endless minipreps! Colony PCR is the ultimate lab hack for molecular cloning, allowing you to rapidly screen bacterial or yeast colonies for your desired DNA insert in just a few hours. This definitive guide cuts through the noise and provides a detailed, step-by-step protocol for success. We dive deep into the one step most scientists get wrong—cell lysis and reveal primer design strategies!

Ready to master apoptosis detection? This comprehensive guide delivers a detailed, step-by-step TUNEL assay protocol, the gold standard for visualizing DNA fragmentation. We've synthesized expert advice from top scientific sources to cover the core principle, a complete protocol for cells and tissues, and the critical troubleshooting tips you need to avoid false positives. Learn how to get reliable, publication-ready data by optimizing fixation, permeabilization, and controls

Unlock the power of genetic engineering with the heat shock transformation method! Learn the precise science behind creating chemically competent cells using calcium chloride and the critical 42 C temperature pulse that forces bacteria to take up life-changing plasmid DNA. Master the detailed protocol steps—from chilling on ice to the crucial outgrowth phase—to achieve maximum transformation efficiency in your molecular cloning experiments and revolutionize protein production

Stop the frustration of watching your precious tissue sections float away during an IHC run. Losing samples is one of the most common and costly failures in a histology lab, but it is almost entirely avoidable. It's not just "bad slides"—your entire protocol, from fixation and processing to your drying time and antigen retrieval technique, plays a crucial role. Learn why a 20-minute bake isn't enough, high-pH buffers problems, and the tricks to ensure your tissue stays firmly

Stop letting poor data visualization undermine your research! Master the creation of powerful, publication-ready grouped bar charts with error bars in GraphPad Prism. This ultimate guide for biomedical scientists details crucial steps, from setting up the correct Grouped Data Table (replicates vs. mean/SEM) to adding transparent individual data points. Elevate your figures and ensure your scientific story is told clearly and accurately for maximum impact.

Unlock the power of your cell viability data! This guide demystifies the two most critical metrics in dose-response analysis: IC 50 ​ and EC 50 ​ . Discover the key difference between measuring inhibition (IC 50 ​ ) and activation (EC 50 ​ ), learn the step-by-step process for calculating these values, and see how they reveal the synergistic power of combination therapies.

Struggling to prove if two compounds are truly better together? Stop guessing and start quantifying. The Checkerboard Assay is the gold-standard method for determining if your products exhibit synergy, additivity, or antagonism. This comprehensive guide provides a detailed, step-by-step protocol for setting up the assay, from cell seeding to data analysis using the Bliss Independence Model.

Struggling with faint or invisible bands in your Western blots? This in-depth guide provides a detailed, step-by-step protocol for detecting low-abundance proteins. We cover critical optimizations from strategic sample preparation and lysate concentration to advanced detection using high-sensitivity ECL substrates. Learn how to choose the right buffers, membranes, and antibodies, and master techniques for enhancing your signal-to-noise ratio.

Unlock the secrets of cellular communication! Our step-by-step guide to the Proximity Ligation Assay (PLA) provides a comprehensive walkthrough of this powerful technique. From sample preparation to data analysis, we'll show you how to visualize protein-protein interactions with stunning clarity. Whether you're a seasoned researcher or new to the field, this guide will equip you with the knowledge and confidence to perform successful PLA experiments.

Unlock the secrets to successful transfection! This comprehensive guide delves into the critical factors that can make or break your experiments. From cell health and nucleic acid quality to choosing the right transfection reagent and optimizing your protocol, we'll walk you through everything you need to know to overcome low efficiency and achieve your research goals. Stop struggling with failed experiments and start getting the results you need.

Unlock the secrets of gene regulation with our comprehensive guide to Chromatin Immunoprecipitation (ChIP). This powerful technique allows you to explore the intricate interactions between proteins and DNA within the cell, providing a "snapshot" of these crucial cellular processes. From cross-linking and cell lysis to DNA purification and analysis, we'll walk you through each step of the ChIP workflow, providing you with the knowledge and confidence to perform successful expe

Unlock the secrets hidden within your paraffin-embedded tissues! This comprehensive guide to immunofluorescence staining provides a detailed, step-by-step protocol for visualizing proteins in FFPE samples. From deparaffinization and antigen retrieval to antibody incubation and imaging, we'll walk you through every stage of the process. Learn how to overcome the challenges of formalin fixation and achieve stunning, publication-quality images. Dive in and illuminate your resear

Struggling with high background on your Western blots? You're not alone. This frustrating issue can obscure your results and leave you wondering where you went wrong. This comprehensive guide delves into the common culprits behind high background, from insufficient blocking and antibody overload to inadequate washing and membrane choice. We'll provide you with practical, actionable solutions to troubleshoot your experiments and achieve the clean, publication-quality blots you

Cell culture contamination is a common yet devastating setback for any scientist. This comprehensive guide will help you identify the culprits, from bacteria to mycoplasma, and provide you with the tools to prevent, detect, and address contamination effectively. Learn how to rescue your research and keep your cell cultures pristine with our expert tips and best practices. Don't let contamination derail your experiments – take control of your cell culture today!

Unleash the power of your data with stunning volcano plots! This comprehensive guide walks you through the process of creating publication-ready volcano plots in R, from setting up your environment to advanced customization techniques. Learn how to visualize differential gene expression data, identify significant genes, and create compelling figures that will make your research stand out.