CytCut 3.5 Is Available Now: The Wound Healing Assay Tool You Asked For, Ready to Ship Today

If you've run a wound healing (scratch) assay by hand, you already know the pain we built CytCut to kill. You drag a pipette tip across a confluent monolayer well after well, the scratches come out uneven, the coefficient of variation runs anywhere from 25% to 60%, and a chunk of your wells are unusable before the experiment even starts. CytCut replaces all of that with a single guided motion that creates clean, uniform, simultaneous scratches across an entire multi-well plate in seconds, no robot, no service contract, no drawer full of spent pipette tips.

Since we opened CytCut, one message kept arriving in our inbox: I don't want to wait. I need this on my bench now. The demand was real and the reason behind it was real, the manual method is genuinely holding experiments back, and every week spent waiting is another week of noisy, hard-to-publish migration data. So we built a version specifically to answer that: get CytCut into researchers' hands faster, without compromising the thing that matters most, the quality of the scratch.

Meet CytCut 3.5: the wound healing assay tool you can order today

CytCut 3.5 is ready to order right now, at $36. It does exactly what CytCut is known for, perfectly uniform, simultaneous scratches across a multi-well plate in a single motion, bringing scratch time down from minutes to seconds and your bad-well rate down with it. This is the same wound healing assay tool and the same core mechanism; what's different is that it's optimized for immediate availability, and we've expanded the format options.

That last point matters: CytCut 3.5 comes in both 24- and 48-well versions. Previously you could only get the 48-well format. Now you choose the plate density that fits your assay.

Here's the honest, complete picture of what 3.5 is and isn't, because you should order with your eyes open:

• Sanitization: fully compatible with UV and 70% alcohol cleaning, the routines most labs already run between assays.

• Not autoclavable. This is the real tradeoff. If your workflow depends on autoclaving the device, 3.5 is not the version for you, and CytCut 4.0 (below) is what you want.

• Working life of 3–6 months of regular use, versus the 6–9 months of the autoclavable version. It's a workhorse for a defined stretch rather than a multi-year fixture.

• Ships immediately. No pre-order window, no waiting for a production run. You order it, it comes.

CytCut 3.5 exists for a specific person: the researcher whose scratch-assay pain is acute right now and who would rather have a reproducibility fix on the bench this month than wait for the premium version. It's not a lesser tool; it's the same scratch quality, tuned for immediate access and a lower entry price.

CytCut 4.0 is still coming, now in two formats

If you want the long-haul device, nothing has changed about the plan, and one thing has improved. The autoclavable CytCut 4.0 is still on track for release at the end of September, and it remains open for pre-order at $99. It's the version built for repeated autoclave cycles and the longer 6–9 month working life.

The upgrade: CytCut 4.0 now comes in both 24- and 48-well formats, where pre-orders previously covered only the 48-well version. Whichever plate density your lab runs, 4.0 will have it.

So there are now two clean paths to standardized scratch assays, and you pick based on what you need:

• Need it now, or want a lower entry price? Order CytCut 3.5 today at $36, 24- or 48-well, ships immediately, UV/alcohol-sanitizable, 3–6 month life.

• Want autoclavability and the longest working life? Pre-order CytCut 4.0 at $99, 24- or 48-well, arriving end of September.

  
  

From clean scratch to clean data: pair the wound healing assay tool with Sophie's Scratch Analyzer

A perfectly uniform wound is only half the workflow. Once you've made the scratches and imaged them, you still have to quantify closure across every well and timepoint, and that analysis step is exactly where the hours pile back up if you're doing it by hand in ImageJ.

This is what Sophie's Scratch Analyzer is built for, and it's the natural other half of CytCut. It's the module of CLYTE's Soφ AI dedicated specifically to scratch-assay data: feed it your images and it measures wound area, closure percentage, and migration rate across your plate, turning what used to be an afternoon of manual tracing into minutes. CytCut standardizes the scratch; the Scratch Analyzer standardizes the analysis. Run them together and the entire wound healing assay, from making the wounds to reporting the migration rate, becomes fast, consistent, and reproducible end to end. If you're ordering CytCut to get cleaner data, Sophie's Scratch Analyzer is how you turn that cleaner data into results without the ImageJ grind.

Order CytCut 3.5 today

The pain of the manual scratch is real, and you told us you didn't want to wait to fix it. Now you don't have to.

• CytCut 3.5 — available now, $36, 24- or 48-well, ships immediately.

• Use code cytcut352673 at checkout for $15 off, valid through July 31.

• Prefer the autoclavable, longest-life version? Pre-order CytCut 4.0 for $99, arriving end of September.

Stop fighting pipette tips, standardize your scratch assays, and get the tool on your bench. Order CytCut now.

FAQ

How is CytCut 3.5 different from CytCut 4.0? CytCut 3.5 is available immediately at $36, is UV- and alcohol-sanitizable (but not autoclavable), and has a 3–6 month working life. CytCut 4.0 is the autoclavable version at $99, with a 6–9 month working life, releasing at the end of September. Both create the same uniform, simultaneous scratches and both now come in 24- and 48-well formats.

Can I autoclave CytCut 3.5? No. CytCut 3.5 is sanitizable with UV light and 70% alcohol, which covers most labs' routine between-assay cleaning, but it is not autoclavable. If autoclaving is essential to your workflow, choose the autoclavable CytCut 4.0.

Which plate formats are available? Both CytCut 3.5 (available now) and CytCut 4.0 (pre-order) come in 24-well and 48-well versions. Previously only the 48-well format was offered, so this is a new option for both.

How long does CytCut 3.5 last? With regular use, CytCut 3.5 has a working life of roughly 3–6 months. The autoclavable CytCut 4.0 lasts longer, about 6–9 months.

How do I analyze the results? Pair CytCut with Sophie's Scratch Analyzer, the module of CLYTE's Soφ AI built specifically for scratch-assay data. It measures wound area, closure percentage, and migration rate across your plate from your images, replacing manual ImageJ tracing and completing the workflow from scratch creation to quantified result.

What's the promo code and when does it expire? Use code cytcut352673 at checkout for $15 off CytCut 3.5, valid through July 31.